Database Query Results : Gallic acid, ,

GA, Gallic acid: Click to Expand ⟱

Features:

Phenolic acid found in gallnuts, sumac, witch hazel, tea leaves, oak bark. Has anitoxidant, antimicrobial and anti-obesity properties.
The GA derivatives include two types: ester and catechin derivatives. The most common ester derivatives of GA are alkyl esters, which are composed mainly of methyl gallate (MG), propyl gallate (PG), octyl gallate (OG), dodecyl gallate (DG), tetradecyl gallate (TG), and hexadecyl gallate (HG), and some of the main catechin derivatives are epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), gallocatechin gallate (GCG), and epigallocatechin gallate (EGCG)

Gallic acid is a naturally occurring polyphenol found in a variety of plant-based foods. Some of the best dietary sources include:

Fruits:
Berries (strawberries, blackberries, blueberries)
Grapes, including red wine (grapes are rich in polyphenols)
Pomegranates and apples
Nuts and Seeds: Walnuts and almonds have been noted to contain GA in their skins
Herbs and Spices: Tea (especially green tea), Sumac and other spices
Other Plants: Gallnuts (from oak trees)

Pathways:
-ROS generation in tumor cells is frequently reported, Antioxidant behavior dominates in normal tissue models -Apoptosis Induction: Activating caspase cascades, Shifting Bax versus Bcl-2, MMP, cyt-c release -Cell Cycle Arrest: typ @ G1 or G2/M checkpoints.
-Anti-inflammatory Effects: inhibiting NF-κB
-reported Angiogenesis Inhibition:
-Modulation of Signaling Pathways: MAPK Pathway, PI3K/Akt Pathway Inhibition, p53 Pathway

Gallic acid exhibits a complex behavior with ROS in cancer cells, acting as both an antioxidant and a pro-oxidant depending on the context and its concentration:

Antioxidant Effects at Low Doses:
-At lower concentrations, gallic acid is typically characterized by its ability to scavenge free radicals, thus reducing oxidative stress.
This antioxidant property may help protect normal cells from DNA damage, reducing the risk of mutations that could lead to cancer.

Pro-oxidant Effects at High Doses: >50-100uM?
-Capable of biphasic redox behavior (antioxidant in normal cells, pro-oxidant in some tumor contexts) -At higher concentrations, GA can exert pro-oxidant effects, generating ROS within cancer cells. Elevated ROS levels can overwhelm the cellular antioxidant defenses of cancer cells, leading to oxidative stress, mitochondrial dysfunction, and ultimately cell death.

Oral bioavailability is moderate but subject to rapid conjugation (glucuronide/sulfate/methylated metabolites). Many cytotoxic in-vitro concentrations are in the 10–100 µM range, often higher than typical plasma levels after dietary intake.

Rank	Pathway / Axis	Cancer / Tumor Context	Normal Tissue Context	TSF	Primary Effect	Notes / Interpretation
1	ROS / Redox modulation (biphasic)	ROS ↑ at higher concentrations (reported); mitochondrial stress ↑	ROS ↓; antioxidant protection	P, R	Redox destabilization (tumor) / buffering (normal)	GA demonstrates dose-dependent redox behavior; pro-oxidant effects are most evident ≥50–100 µM in vitro.
2	Nrf2 / ARE antioxidant response	Context-dependent; may support stress adaptation	Nrf2 ↑; HO-1 ↑; GSH ↑	R, G	Redox regulation	Activation common in non-malignant oxidative stress models; tumor implications vary and may affect therapy sensitivity.
3	NF-κB inflammatory signaling	NF-κB ↓; COX-2, IL-6, TNF-α ↓ (reported)	Inflammation tone ↓	R, G	Anti-inflammatory + anti-survival transcription	One of the more consistent signaling findings across inflammatory and tumor models.
4	Intrinsic apoptosis (mitochondrial; p53-related)	ΔΨm ↓; Bax ↑; Bcl-2 ↓; caspases ↑; cyt-c ↑ (reported)	↔ (limited activation)	G	Cell death execution	Often ROS-mediated; p53 activation reported in several systems.
5	Cell-cycle checkpoints (G1 / G2-M)	Cell-cycle arrest ↑ (Cyclin/CDK modulation)	↔	G	Cytostasis	Phase varies by tumor model; commonly G1 or G2/M.
6	PI3K → AKT (± mTOR)	PI3K/AKT signaling ↓ (reported; model-dependent)	↔	R, G	Growth/survival suppression	Likely secondary to redox and inflammatory signaling modulation.
7	MAPK pathways (ERK / JNK / p38)	JNK/p38 activation; ERK modulation (context-dependent)	↔	P, R, G	Stress signaling reprogramming	Often linked to ROS-mediated apoptosis pathways.
8	Angiogenesis signaling (VEGF)	VEGF ↓ (reported in some models)	↔	G	Anti-angiogenic modulation	Evidence present but less consistent than redox and NF-κB effects.
9	Invasion / metastasis (MMPs / EMT)	MMP2/MMP9 ↓; migration ↓ (reported)	↔	G	Anti-invasive phenotype	Likely downstream of NF-κB and MAPK modulation.
10	Bioavailability constraint (phase II metabolism)	Rapid glucuronidation/sulfation; free GA low	—	—	Translation constraint	Plasma levels after dietary intake are generally below many in-vitro cytotoxic concentrations.

Time-Scale Flag (TSF): P / R / G

P: 0–30 min (rapid redox interactions)
R: 30 min–3 hr (acute signaling and stress-response shifts)
G: >3 hr (gene-regulatory adaptation and phenotype outcomes)

Scientific Papers found: Click to Expand⟱

947-

GA,

Gallic acid, a phenolic compound, exerts anti-angiogenic effects via the PTEN/AKT/HIF-1α/VEGF signaling pathway in ovarian cancer cells

in-vitro,

Ovarian,

OVCAR-3

0, 5, 10, or 20 μM GA for 24 h

in-vitro,

Melanoma,

A2780S

in-vitro,

Nor,

IOSE364

40uM

Notably, GA did not have a significant inhibitory effect on the normal cell line (IOSE-364) at 40 μM.   ****

Human,

NA,

8-10uM / 800mg oral GA

~8–10 μM of GA was detected in the serum of healthy volunteers, after oral intake of a combination of a dietary herbal supplement and 800 mg GA

TumCG↓, VEGF↓, angioG↓, p‑Akt↓, Hif1a↓, PTEN↑, BioAv↑, *toxicity↓,

987-

GA,

Targeting Aerobic Glycolysis: Gallic Acid as Promising Anticancer Drug

in-vitro,

GBM,

AMGM

1-100uM

1uM->90% growth inhibition @72hr

in-vitro,

Cerv,

HeLa

in-vitro,

BC,

MCF-7

LDH↓, TumCG↓,

997-

GA,

The Inhibitory Mechanisms of Tumor PD-L1 Expression by Natural Bioactive Gallic Acid in Non-Small-Cell Lung Cancer (NSCLC) Cells

in-vitro,

Lung,

A549

400 μM @48hrs

in-vitro,

Lung,

H292

100 μM@48hrs

in-vitro,

Nor,

HUVECs

400 μM GA inducing around 8% cell death which indicated that this concentration does not make much toxicity in normal cells  ****

PD-L1↓, p‑EGFR↓, p‑PI3K↓, p‑Akt↓, P53↑, miR-34a↑, *toxicity↓,

1065-

GA,

Gallic acid, a phenolic acid, hinders the progression of prostate cancer by inhibition of histone deacetylase 1 and 2 expression

vitro+vivo,

Pca,

mice

tumCV↓, MMP↓, DNAdam↑, HDAC1↓, HDAC2↓, PCNA↓, cycD1/CCND1↓, cycE1↓, P21↑, TumVol↓,

1086-

GA,

Anti-leukemic effects of gallic acid on human leukemia K562 cells: downregulation of COX-2, inhibition of BCR/ABL kinase and NF-κB inactivation

in-vitro,

AML,

K562

tumCV↓, TumCCA↑, P21↑, p27↑, cycD1/CCND1↓, cycE/CCNE↓, Bax:Bcl2↑, Cyt‑c↑, cl‑PARP↓, DNAdam↑, Casp3↑, FASN↓, Casp8↑,

1091-

GA,

Gallic acid reduces cell viability, proliferation, invasion and angiogenesis in human cervical cancer cells

in-vitro,

Cerv,

HeLa

in-vitro,

Cerv,

HTB-35

tumCV↓, TumCP↓, ADAM17↓, EGFR↓, p‑Akt↓, p‑ERK↓,

1115-

GA,

Gallic acid alleviates gastric precancerous lesions through inhibition of epithelial mesenchymal transition via Wnt/β-catenin signaling pathway

in-vivo,

GC,

GES-1

mice,induced precancerous lesions of gastric cancer cells (MC)

TumCP↓, TumCCA↑, Wnt/(β-catenin)↓, EMT↓,

1283-

GA,

immuno,

Gallic acid induces T-helper-1-like Treg cells and strengthens immune checkpoint blockade efficacy

vitro+vivo,

CRC,

p‑STAT3↓, Treg lymp↓, FOXP3↓, CD8+↑, IFN-γ↑,

1300-

GA,

PacT,

carbop,

Gallic acid potentiates the apoptotic effect of paclitaxel and carboplatin via overexpression of Bax and P53 on the MCF-7 human breast cancer cell line

in-vitro,

BC,

MCF-7

TumCCA↑, Apoptosis↑, P53↑, BAX↑, Casp3↑, Bcl-2↓,

1624-

GA,

Anticancer Effect of Pomegranate Peel Polyphenols against Cervical Cancer

in-vitro,

Cerv,

ROS↑, Dose∅, MMP↓, GSH↑,

1773-

GA,

Impact of Gallic Acid on Gut Health: Focus on the Gut Microbiome, Immune Response, and Mechanisms of Action

Review,

Var,

antiOx↑, AntiCan↑, Inflam↑, GutMicro↑, BioAv↝, BioAv↓, BioAv↑, TumMeta↓,

* indicates research on normal cells as opposed to diseased cells
Total Research Paper Matches: 11

Pathway results for Effect on Cancer / Diseased Cells:

Infection & Microbiome ⓘ

CD8+↑, 1,
Total Targets: 60

Pathway results for Effect on Normal Cells:

Functional Outcomes ⓘ

toxicity↓, 2,
Total Targets: 1

Query results interpretion may depend on "conditions" listed in the research papers.
Such Conditions may include : 
  -low or high Dose
  -format for product, such as nano of lipid formations
  -different cell line effects
  -synergies with other products 
  -if effect was for normal or cancerous cells

Filter Conditions: Pro/AntiFlg:%  IllCat:%  CanType:%  Cells:%  prod#:82  Target#:%  State#:%  Dir#:%
  wNotes=0  sortOrder:rid,rpid

Database Query Results : Gallic acid, ,

Pathway results for Effect on Cancer / Diseased Cells:

Redox & Oxidative Stress ⓘ

Mitochondria & Bioenergetics ⓘ

Core Metabolism/Glycolysis ⓘ

Cell Death ⓘ

Transcription & Epigenetics ⓘ

DNA Damage & Repair ⓘ

Cell Cycle & Senescence ⓘ

Proliferation, Differentiation & Cell State ⓘ

Migration ⓘ

Angiogenesis & Vasculature ⓘ

Immune & Inflammatory Signaling ⓘ

Cellular Microenvironment ⓘ

Drug Metabolism & Resistance ⓘ

Clinical Biomarkers ⓘ

Functional Outcomes ⓘ

Infection & Microbiome ⓘ

Pathway results for Effect on Normal Cells:

Functional Outcomes ⓘ

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