Database Query Results : , , TOPflash

TOPflash, TOPflash: Click to Expand ⟱
Source:
Type: reporter construct
TOPflash is not an endogenous gene or protein but rather a reporter construct that is widely used in experimental studies to assess the transcriptional activity of the canonical Wnt/β-catenin signaling pathway.
By comparing the activity of TOPflash with that of a control reporter (such as FOPflash, which contains mutated TCF binding sites), investigators assess the specific contribution of Wnt/β-catenin signaling in cancer cell lines or experimental models.

Instead, the prognostic relevance lies in the fact that high activity of the canonical Wnt pathway—often inferred from assays using TOPflash—has been associated with more aggressive cancer phenotypes and poorer prognoses in several tumor types.
Scientific Papers found: Click to Expand⟱
1500- SFN,    A novel mechanism of chemoprotection by sulforaphane: inhibition of histone deacetylase
- in-vitro, Nor, HEK293 - in-vitro, CRC, HCT116
HDAC↓, P21↑, TOPflash↑,
1820- VitK3,    Vitamin K3 (menadione) suppresses epithelial-mesenchymal-transition and Wnt signaling pathway in human colorectal cancer cells
- in-vitro, CRC, SW480 - in-vitro, CRC, SW-620
selectivity↑, TumCI↓, TumCMig↓, EMT↓, E-cadherin↑, ZO-1↑, N-cadherin↓, Vim↓, Zeb1↓, MMP2↓, MMP9↓, TOPflash↓, β-catenin/ZEB1↓, p300↓, cycD1/CCND1↓, TumCCA↑,

* indicates research on normal cells as opposed to diseased cells
Total Research Paper Matches: 2

Pathway results for Effect on Cancer / Diseased Cells:


Cell Cycle & Senescence ⓘ

cycD1/CCND1↓, 1,   P21↑, 1,   TumCCA↑, 1,  

Proliferation, Differentiation & Cell State ⓘ

EMT↓, 1,   HDAC↓, 1,   p300↓, 1,   TOPflash↓, 1,   TOPflash↑, 1,  

Migration ⓘ

E-cadherin↑, 1,   MMP2↓, 1,   MMP9↓, 1,   N-cadherin↓, 1,   TumCI↓, 1,   TumCMig↓, 1,   Vim↓, 1,   Zeb1↓, 1,   ZO-1↑, 1,   β-catenin/ZEB1↓, 1,  

Drug Metabolism & Resistance ⓘ

selectivity↑, 1,  
Total Targets: 19

Pathway results for Effect on Normal Cells:


Total Targets: 0

Scientific Paper Hit Count for: TOPflash, TOPflash
Query results interpretion may depend on "conditions" listed in the research papers.
Such Conditions may include : 
  -low or high Dose
  -format for product, such as nano of lipid formations
  -different cell line effects
  -synergies with other products 
  -if effect was for normal or cancerous cells

Filter Conditions: Pro/AntiFlg:%  IllCat:%  CanType:%  Cells:%  prod#:%  Target#:1113  State#:%  Dir#:%
  wNotes=0  sortOrder:rid,rpid

 

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